Sequences from confirmation amplicon


Confirmation sequences for line 873G03 and hit At2g01940 are shown.
Line ID 873G03
Locus At2g01940 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr2:433098
Primers used for PCR 41sf - 8474, show primer details
   
Sequence Name 59-K116491-0022-873-G03C-41sf
GenBank Accession LN510084 [GenBank]
Sequencing primer gene specific primer, 41sf: 5'-AGCATGACAAGGATTATGGTGAAG-3'
Sequence (A. th genome BLAST matches underlined) 
>59-K116491-0022-873-G03C-41sf
TTTCTTCCCTCTATGTTGTTGTCTCTTTAAGAAGCTTCCATGGAACTTTGTGACGTCTTC
TATGCATCTGGAGATTCTGGTCTCTTTGAAACCCTTGGTTACAGATCTCACATATGTATC
TGTCTGATTCAAGAAGAGTTCTTGGTGATAAAGACACAACTTCTGCATCTGGATCTGAAA
AGGAGATAAGGCAAAACCCATTAATAAAGAAAAAGGATATATTCAATTGTAAATGGCTTC
ATGTCCGGGAAATCTACATGGATCAGCAATGAGTATGATGGTCAATATGGAGAAAAAGAA
AGAGTAATTACCAATTTTTTTTCAATTCAAAAATGTAGATGTCCGCAAAGTTATTATAAT
   
Sequence Name 27-K116490-0022-873-G03C-8409
GenBank Accession LN498554 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>27-K116490-0022-873-G03C-8409
CATTTACATTGAATATATCCTTTTTCTTTATTAATGGGTTTTGCCTTATCTCCTTTTCAG
ATCCAGATGCAGAAGTTGTGTCTTTATCACCAAGAACTCTTCTTGAATCAGACAGATACA
TATGTGAGATCTGTAACCAAGGGTTTCAAAGAGACCAGAATCTCCAGATGCATAGAAGAC
GTCACAAAGTTCCATGGAAGCTTCTTAAAAGAGACAACAACATAGAGGTGAAGAAACGAG
TCTATGTTTGCCCTGAACCCACTTGCCTTCACCATAATCCTTGTCATGCTA

Last Updated on 06.12.2018 16:11