Genotyping and primer details


Genotyping and primer details for 873G03 - At2g01940
Line ID 873G03
Locus At2g01940 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Graphic view of Chr2:433098 of line 873G03 Chr2:433098
   
Insertion allele amplicon 8474 - 41sf
T-DNA primer 8474: 5'-ATAATAACGCTGCGGACATCTACATTTT-3'
Locus specific primer 41sf: 5'-AGCATGACAAGGATTATGGTGAAG-3'
Sequences from confirmation amplicon show confirmation sequences
Genomic end of ins. amplicon Chr2:433354
   
wt amplicon (over InsPos) 13tc - 41sf
wt amplicon start Chr2:432674
wt amplicon end Chr2:433354
wt amplicon size 681 bp
Locus specific primer 41sf: 5'-AGCATGACAAGGATTATGGTGAAG-3'
2nd locus specific primer 13tc: 5'-TGTCCAACAAGAACACAAACACAT-3'
   
Note The data concerning the primers refer to the experimental conditions used in the initial analysis of the alleles. In general, we used 8474 in the PCR and 8409 for sequencing. See the confirmation strategy page for details. For genotyping in the context of DUPLO, we used 8409 as T-DNA primer in PCRs. See the DUPLOdb help page for details.

Last Updated on 06.12.2018 16:11