Sequences from confirmation amplicon


Confirmation sequences for line 880B04 and hit At5g10330 are shown.
Line ID 880B04
Locus At5g10330 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr5:3249786
Primers used for PCR 37vh - 8474, show primer details
   
Sequence Name 46-K117932-0022-880-B04C-37vh
GenBank Accession LN505997 [GenBank]
Sequencing primer gene specific primer, 37vh: 5'-AGTGAGTCATAGAAAAACCGAAGC-3'
Sequence (A. th genome BLAST matches underlined) 
>46-K117932-0022-880-B04C-37vh
TACTTAGGGTTATCTCCATTTGCCCCTCTTCCTGTCAACTTTCAGGTATTACTTGTCAAT
AAGATGTGAAATTGAGTATGTGCATCAGATGTTTCCTCTAGATTGAACTGATTCCACCTT
TGATATGCGCAGACACGAAAACTGATGGGTGTGATCAATGTTCAGGGCTCTCCATCGTTC
TCTATTCATTCTTCAGAGTCAAATCTTCGAAAGTCTCCCCAATTGATCAGGATATATTCA
ATTGTAAATGGCTTCATGTCCGGGAAATCTACATGGATCAGCAATGAGTATGATGGTCAA
TATGGAGAAAAAGAAAGAGTAATTACCAATTTTTTTTCAATTCAAAAATGTAGATGTCCG
CAGCGTTATTAT
   
Sequence Name 06-K117931-0022-880-B04C-8409
GenBank Accession queueing for submission to ENA/GenBank
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>06-K117931-0022-880-B04C-8409
AGCTTCGGTTTTTCTATGACTCACTAGGAGAAAAAGAAAGAGTAATTACCAATTTTTTTT
CAATTCAAAAATGTAGATGTCCGCAGCGTTATA

Last Updated on 06.12.2018 16:11