Genotyping and primer details


Genotyping and primer details for 880B04 - At5g10330
Line ID 880B04
Locus At5g10330 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Graphic view of Chr5:3249786 of line 880B04 Chr5:3249786
   
Insertion allele amplicon 8474 - 37vh
T-DNA primer 8474: 5'-ATAATAACGCTGCGGACATCTACATTTT-3'
Locus specific primer 37vh: 5'-AGTGAGTCATAGAAAAACCGAAGC-3'
Sequences from confirmation amplicon show confirmation sequences
Genomic end of ins. amplicon Chr5:3250077
   
wt amplicon (over InsPos) 37vu - 37vh
wt amplicon start Chr5:3250077
wt amplicon end Chr1:27067811
wt amplicon size 863 bp
Locus specific primer 37vh: 5'-AGTGAGTCATAGAAAAACCGAAGC-3'
2nd locus specific primer 37vu: 5'-ATTCAGACTCCAAACCAGAGTCTT-3'
   
Note The data concerning the primers refer to the experimental conditions used in the initial analysis of the alleles. In general, we used 8474 in the PCR and 8409 for sequencing. See the confirmation strategy page for details. For genotyping in the context of DUPLO, we used 8409 as T-DNA primer in PCRs. See the DUPLOdb help page for details.

Last Updated on 06.12.2018 16:11