Sequences from confirmation amplicon


Confirmation sequences for line 899C10 and hit At4g26420 are shown.
Line ID 899C10
Locus At4g26420 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr4:13351366
Primers used for PCR 28vi - 8474, show primer details
   
Sequence Name 52-K117932-0022-899-C10C-28vi
GenBank Accession LN507946 [GenBank]
Sequencing primer gene specific primer, 28vi: 5'-AATTTAATTTGTATCGGCCAAAAA-3'
Sequence (A. th genome BLAST matches underlined) 
>52-K117932-0022-899-C10C-28vi
TTAAATTATCGTTTCTTCTAATGTTAATGAACAATATGTCAAAAAATTTATAAAGTTCTA
TTATGATATTCTTCAAAAACATTTTTGTTCCATGACCGAACCAACTTAATTTATATAACT
GATTATTGTTTTAATGTATATATATCTTATGGGTACAGAAATGTTTCAATTTACGTTTTT
TGTTTTTTAAATGTTTCATTTCTTTGTATTGGATATGATAGGTACCTGAAAAAGTGATGG
AGAAAGGATCGAAGTCATGGAACAAGGGAGGGGTGTGGATTGAAGGAGCAGAAAAAGAAG
TCGTGGAGGCATACGCGGAGCAAGCAGACAAGGACTTAGTCGAGTTCTTGAAATGTCGCA
AAGAGGAGATTGTGGTAGGAGGAGTGTTGTTTATGCTGATGGGTGGTCGACCTTCTGGCT
CCGTGAACCAAATCGGTGATCCTGATTCGAGTCTCAAACACCCTTTCACAACTTTGATGG
ATCAAGTAGATTATATTATAAATGGCTTCATGTCCGGGAAATCTACATGGATCAGCAATG
AGTATGATGGTCAATATGGAGAAAAAGAAAGAGTAATTACCAATTTTTTTTCAATTCAAA
AATGTAGATGTCCGCA
   
Sequence Name 12-K117931-0022-899-C10C-8409
GenBank Accession LN491777 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>12-K117931-0022-899-C10C-8409
TTCGAATCAGGATCACCGATTTGGTTCACGGAGCCAGAAGGTCGACCACCCATCAGCATA
AACTACACTCCTCCTACCGCAATCTGCTCTTTGGGACATTTCAAGAACTCGACTAAGTCC
TGTGTCTGCTTGCTCCGCGTAGGCCTCCACGAGTTCTTTTTCTGCTCCTTGAATCCACAC
CGCTCCCGTGTTCCATGACTTCGATCCGATTCTCCATCACGTATTCAGGTACGGGTGAGG
ACCGATAGAGCGAAATGAAACGTTTAGAAGGCAAACGACGTACATTGAAACATTGCTGTA
CCCATGATATATATATGCATTAAAACAAAAATCGGGTATAGAAAGAAGGTTGGTTCGGTG
ATGGAAGGAAAATGTTCTGGAAGAGTATCATAATACAACGATATAGATTTTTGGACATAT
CGCTCATTAACATTAGAAGAAACGATAGTTTAACTTTTTGTTTAGGTCTACTAGTCTAGT
AATCTTCAAATCATTTTTTGGCCGATACAGATTAAATTAGGTGACTGATTATGGTTTTTA
CGCAATTTTTTCTCAATGGGTAAAGGTATGTGTCC

Last Updated on 06.12.2018 16:11