Genotyping and primer details


Genotyping and primer details for 899C10 - At4g26420
Line ID 899C10
Locus At4g26420 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Graphic view of Chr4:13351366 of line 899C10 Chr4:13351366
   
Insertion allele amplicon 8474 - 28vi
T-DNA primer 8474: 5'-ATAATAACGCTGCGGACATCTACATTTT-3'
Locus specific primer 28vi: 5'-AATTTAATTTGTATCGGCCAAAAA-3'
Sequences from confirmation amplicon show confirmation sequences
Genomic end of ins. amplicon Chr4:13351916
   
wt amplicon (over InsPos) 55vy - 28vi
wt amplicon start Chr4:13350650
wt amplicon end Chr4:13351916
wt amplicon size 1267 bp
Locus specific primer 28vi: 5'-AATTTAATTTGTATCGGCCAAAAA-3'
2nd locus specific primer 55vy: 5'-GTTTCACTTTTCCGATCAATTCTT-3'
   
Note The data concerning the primers refer to the experimental conditions used in the initial analysis of the alleles. In general, we used 8474 in the PCR and 8409 for sequencing. See the confirmation strategy page for details. For genotyping in the context of DUPLO, we used 8409 as T-DNA primer in PCRs. See the DUPLOdb help page for details.

Last Updated on 06.12.2018 16:11