Sequences from confirmation amplicon


Confirmation sequences for line 966C01 and hit At2g16770 are shown.
Line ID 966C01
Locus At2g16770 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr2:7280577
Primers used for PCR 23lg - 8474, show primer details
   
Sequence Name 74-K113934-0022-966-C01C-23lg
GenBank Accession LN513118 [GenBank]
Sequencing primer gene specific primer, 23lg: 5'-AGCTGAGGTTACTAGGCTCAAGTG-3'
Sequence (A. th genome BLAST matches underlined) 
>74-K113934-0022-966-C01C-23lg
GATGGAAGATTGGTGCGTTTCCTTATAGAAACCTGCGGTTACAAACGTGCCTTACTCATA
CATGATGCATCCTTGCAATATGCAATGTGATGTTGATAACTTGTACTGCCTTCAAAATGG
GAATAATGGAGAAGGTGCATCGATGAATGAGCAAGGGCTAAATGGTTGTGAGTTTGATCA
GCTAGAATGCTTGGCTAATCAGAATTTAGCTGGCAAGGAGATCCCTGTTTGTAGTAATGG
AATTGGAACATTCACTGTCAATGGATCCGGCGTTAATAAGAGAAACAATTGTAGTTACCA
ATTTATATTCAATTGTAAATGGCTTCATGTCCGGGAAATCTACATGGATCAGCAATGAGT
ATGATGGTCAATATGGAGAAAAAGAAAGAGTAATTACCAATTTTTTTTCAATTCAAAAAT
GTAGATGTCCGCACGAGTTATTA
   
Sequence Name 39-K113933-0022-966-C01C-8409
GenBank Accession LN503426 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>39-K113933-0022-966-C01C-8409
CATTTCATTGAATAAAATTGGTAACTACATTGTTTCTCTTATTAACGCCGGATCCATTGA
CAGTGAATGTTCCAATTCCATTACTACAAACAGGGATCTCCTTGCCAGCTAAATTCTGAT
TAGCCAAGCATTCTAGCTGATCAAACTCACAACCATTTAGCCCTTGCTCATTCATCGATG
CACCTTCTCCATTATTCCCATTTTGAAGGCAGTACAAGTTATCAACATCACATTGCATAT
TGCAAGGATGCATCATGTATGAGTAAGGCACGTTTGTAACCGCAGGTTTCTGATAAGGAA
ACGCACCAATCTCTCCATCAATTCTTCCTCTTATATCAACAAGCAAACACTTGAGCCTAG
AAACCTCAGCTA

Last Updated on 06.12.2018 16:11