Genotyping and primer details


Genotyping and primer details for 966C01 - At2g16770
Line ID 966C01
Locus At2g16770 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Graphic view of Chr2:7280577 of line 966C01 Chr2:7280577
   
Insertion allele amplicon 8474 - 23lg
T-DNA primer 8474: 5'-ATAATAACGCTGCGGACATCTACATTTT-3'
Locus specific primer 23lg: 5'-AGCTGAGGTTACTAGGCTCAAGTG-3'
Sequences from confirmation amplicon show confirmation sequences
Genomic end of ins. amplicon Chr2:7280239
   
wt amplicon (over InsPos) 67rd - 23lg
wt amplicon start Chr2:7280239
wt amplicon end Chr2:7280876
wt amplicon size 638 bp
Locus specific primer 23lg: 5'-AGCTGAGGTTACTAGGCTCAAGTG-3'
2nd locus specific primer 67rd: 5'-TTAACTACCAACTTGAAAATGGGG-3'
   
Note The data concerning the primers refer to the experimental conditions used in the initial analysis of the alleles. In general, we used 8474 in the PCR and 8409 for sequencing. See the confirmation strategy page for details. For genotyping in the context of DUPLO, we used 8409 as T-DNA primer in PCRs. See the DUPLOdb help page for details.

Last Updated on 06.12.2018 16:11