SimpleSearch - Line and FST details
Line specific information
| Line ID | 009A02 |
| Vector Used | pAC106 |
| Line Availability | available as T3 set from NASC (N400770) |
| Segregation Analysis | 100:95:85 |
| Confirmed for Hit | At1g60510 MPL12 |
| Parent of DUPLO pair | none |
| Parent of pair(s) | none |
| Note |
Gene hit At1g60510
| Sequence (A. th genome BLAST matches underlined) | >09-K014859-022-009-A02-8409 TTGATCCATGGTAGATTTCCCGGACATGAANGCATTTACAATTGAATTATCAAGTGTTGA GCCCAATGAACGAAAATTATCAAGTTACGCCTTTAGTCAAAATTCCAAACTTCACCACAA AAGAATTCAGCGCATTGGAACCAATAAAATATTCAAATACACATCTTAAGAGAGACGTTT TAGTTTCAACTACGAGCTATGGGATCCTATCATGTGGTGAGANGNATTTGNANNNNGNCA AATGGATTTGTACCTACATAAATAGTGCAAGCCAGTGAGTTTTGGAAGCTATGAAACAAT TCGTCGCTTTTGGGCTCAAAGTTATCGGAGAATGCCTGCTTCTA |
| GenBank Accession | AL751940 [GenBank] |
| Graphic View | 
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| Predicted Position of Insertion | Chr1:22294336 - go to primer design |
| BLAST e Value | 2e-77 |
| Hit Clone Code (BAC ID) | F8A5 |
| Hit Gene Code | At1g60510 [Araport] [TAIR] [MIPS] [SIGnAL] |
| Gene Annotation | |
| Insertion Classification | 5' region |
| Confirmation Status | confirmed, show confirmation sequences |
Gene hit MPL12
| Sequence (A. th genome BLAST matches underlined) | >01-K020160-022-009-A02t-8409 AGGTATCCTCTTTATTGTGAGGTTGTATTTGTAAAAAGTCAAATGTATTTGTACCTACGT AAATAGTGCAAGCCAGTGAGTTTTGGAAGCTATAAAACAATTCGTCGCTTTTGGTCTCAA AGTTATCGGAGAATGTCTACTTCTATGGGATCCTCCCTATAGTGAGANNNNNNNNNNG |
| GenBank Accession | BX530726 [GenBank] |
| Graphic View |
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| Predicted Position of Insertion | Chr5:18779518 - go to primer design |
| BLAST e Value | 3e-64 |
| Hit Clone Code (BAC ID) | MPL12 |
| Insertion Classification | Genome Hit |
| Confirmation Status | confirmed, show confirmation sequences |
| Primer and wt-amplicons | show primer details |
| Other FSTs Supporting this Hit | CR769809 [GenBank] CR769808 [GenBank] FR799960 [GenBank] FR799961 [GenBank] FR799961 [GenBank] |
Gene hit T20E23
| Sequence (A. th genome BLAST matches underlined) | >57-K014860-022-009-A02-8409 NTGATCCATGTAGATTNTCCCGGACATGAANGCCTTTACAATTGAATATATCCTGGATGT AATCTACGACCAAATCGATTCTCAAATCTTCTTTCAATAGTTTATATAAAGGGTAAAAGT ATTTTTTGGCGGGCATAATTATCGCTTTTTGCCATTTTTACCCCCGTACCATTACAGACA AAATAATAAAGAGCTCTGGGGCCAGTATGATAAATTGGTATAGGGACCTACCATACACTT TCTCCCTTTTTCTTTTTGATAGCGATTGCGCCAATATAAAAAGGGTATTTCCAGTGGTGG TTCTGTCAATTAGACAACAACAACAACAGCAACAACATGCCTCAGCTCCTGCAAACTTCA CCTCATTTGGAATGGTTCCTATGGGATCCT |
| GenBank Accession | AL751941 [GenBank] |
| Graphic View | 
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| Predicted Position of Insertion | Chr3:18750322 - go to primer design |
| BLAST e Value | 3e-35 |
| Hit Clone Code (BAC ID) | T20E23 |
| Insertion Classification | Genome Hit |
| Confirmation Status | failed |
|
Last Updated on 10.06.2021 13:37 |
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