Genotyping and primer details


Genotyping and primer details for SALK_113003 - At1g15720
Line ID SALK_113003
Locus At1g15720 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Graphic view of Chr1:5406128 of line SALK_113003 Chr1:5406128
   
Insertion allele amplicon LBb3 - 53ew
T-DNA primer LBb3: 5'-ATTTTGCCGATTTCGGAAC-3'
Locus specific primer 53ew: 5'-TAAGAAACCTCTTTCGAGCTTCTG-3'
Sequences from confirmation amplicon show confirmation sequences
Genomic end of ins. amplicon Chr1:5406726
   
wt amplicon (over InsPos) 50gc - 53ew
wt amplicon start Chr1:5405824
wt amplicon end Chr1:5406726
wt amplicon size 903 bp
Locus specific primer 53ew: 5'-TAAGAAACCTCTTTCGAGCTTCTG-3'
2nd locus specific primer 50gc: 5'-ATTGGATCAACATTGTATTGCTCA-3'
   
Note The data concerning the primers refer to the experimental conditions used in the initial analysis of the alleles. However, in the course of the project we replaced the primer R204 with LBb3. See the DUPLOdb help page for details.

Last Updated on Thursday, 06 December 2018 16:11