Genotyping and primer details


Genotyping and primer details for SALK_008497c - At1g80440
Line ID SALK_008497c
Locus At1g80440 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Graphic view of Chr1:30242151 of line SALK_008497c Chr1:30242151
   
Insertion allele amplicon LBb3 - 50ew
T-DNA primer LBb3: 5'-ATTTTGCCGATTTCGGAAC-3'
Locus specific primer 50ew: 5'-TTAACCGTTTTTGCCCATACTAAC-3'
Sequences from confirmation amplicon show confirmation sequences
Genomic end of ins. amplicon Chr1:30241427
   
wt amplicon (over InsPos) 30gc - 50ew
wt amplicon start Chr1:30241427
wt amplicon end Chr1:30242335
wt amplicon size 909 bp
Locus specific primer 50ew: 5'-TTAACCGTTTTTGCCCATACTAAC-3'
2nd locus specific primer 30gc: 5'-GTCTTCTGACACGTCATACACCAT-3'
   
Note The data concerning the primers refer to the experimental conditions used in the initial analysis of the alleles. However, in the course of the project we replaced the primer R204 with LBb3. See the DUPLOdb help page for details.

Last Updated on Thursday, 06 December 2018 16:11