Genotyping and primer details


Genotyping and primer details for 718D10 - At2g41960
Line ID 718D10
Locus At2g41960 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Graphic view of Chr2:17514609 of line 718D10 Chr2:17514609
   
Insertion allele amplicon 8474 - 16ie
T-DNA primer 8474: 5'-ATAATAACGCTGCGGACATCTACATTTT-3'
Locus specific primer 16ie: 5'-AGTCTCTTGGTCTTCAGCTTCAAC-3'
Sequences from confirmation amplicon show confirmation sequences
Genomic end of ins. amplicon Chr2:17514814
   
wt amplicon (over InsPos) 06jf - 16ie
wt amplicon start Chr2:17513915
wt amplicon end Chr2:17514814
wt amplicon size 900 bp
Locus specific primer 16ie: 5'-AGTCTCTTGGTCTTCAGCTTCAAC-3'
2nd locus specific primer 06jf: 5'-TTTACCTGATGAGTCCACATTGAG-3'
   
Note The data concerning the primers refer to the experimental conditions used in the initial analysis of the alleles. In general, we used 8474 in the PCR and 8409 for sequencing. See the confirmation strategy page for details. For genotyping in the context of DUPLO, we used 8409 as T-DNA primer in PCRs. See the DUPLOdb help page for details.

Last Updated on Thursday, 06 December 2018 16:11