Genotyping and primer details


Genotyping and primer details for SALK_015570 - At4g22750
Line ID SALK_015570
Locus At4g22750 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Graphic view of Chr4:11949989 of line SALK_015570 Chr4:11949989
   
Insertion allele amplicon LBb3 - 15im
T-DNA primer LBb3: 5'-ATTTTGCCGATTTCGGAAC-3'
Locus specific primer 15im: 5'-GCTGTTGGTTTCTTGTGTGTTTTA-3'
Sequences from confirmation amplicon show confirmation sequences
Genomic end of ins. amplicon Chr4:11950739
   
wt amplicon (over InsPos) 08kj - 15im
wt amplicon start Chr4:11949533
wt amplicon end Chr4:11950739
wt amplicon size 1207 bp
Locus specific primer 15im: 5'-GCTGTTGGTTTCTTGTGTGTTTTA-3'
2nd locus specific primer 08kj: 5'-TTAAAGCTTTCATTGTGTTCGATG-3'
   
Note The data concerning the primers refer to the experimental conditions used in the initial analysis of the alleles. However, in the course of the project we replaced the primer R204 with LBb3. See the DUPLOdb help page for details.

Last Updated on Thursday, 06 December 2018 16:11