Genotyping and primer details


Genotyping and primer details for 654A08 - At1g07550
Line ID 654A08
Locus At1g07550 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Graphic view of Chr1:2323919 of line 654A08 Chr1:2323919
   
Insertion allele amplicon 8474 - EU33
T-DNA primer 8474: 5'-ATAATAACGCTGCGGACATCTACATTTT-3'
Locus specific primer EU33: 5'-AATATTTCTACACACCTATGGCGG-3'
Sequences from confirmation amplicon show confirmation sequences
Genomic end of ins. amplicon Chr1:2323508
   
Insertion allele amplicon CR3S - SP09
T-DNA primer CR3S: 5'-TTGAGCATATAAGAAACCCTTAGT-3'
Locus specific primer SP09: 5'-CTTAAGTGGAAACAAACTTAGCGG-3'
Sequences from confirmation amplicon show confirmation sequences
Genomic end of ins. amplicon Chr1:2324491
   
wt amplicon (over InsPos) SP09 - EU33
wt amplicon start Chr1:2323508
wt amplicon end Chr1:2324491
wt amplicon size 984 bp
Locus specific primer EU33: 5'-AATATTTCTACACACCTATGGCGG-3'
2nd locus specific primer SP09: 5'-CTTAAGTGGAAACAAACTTAGCGG-3'
   
Note The data concerning the primers refer to the experimental conditions used in the initial analysis of the alleles. In general, we used 8474 in the PCR and 8409 for sequencing. See the confirmation strategy page for details. For genotyping in the context of DUPLO, we used 8409 as T-DNA primer in PCRs. See the DUPLOdb help page for details.

Last Updated on 06.12.2018 16:11