Genotyping and primer details


Genotyping and primer details for 428E05 - At1g10560
Line ID 428E05
Locus At1g10560 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Graphic view of Chr1:3486030 of line 428E05 Chr1:3486030
   
Insertion allele amplicon 8474 - BB79
T-DNA primer 8474: 5'-ATAATAACGCTGCGGACATCTACATTTT-3'
Locus specific primer BB79: 5'-GACACAAGTTCAAAATCAGACCAA-3'
Sequences from confirmation amplicon show confirmation sequences
Genomic end of ins. amplicon Chr1:3486503
   
wt amplicon (over InsPos) 04cr - BB79
wt amplicon start Chr1:3485471
wt amplicon end Chr1:3486503
wt amplicon size 1033 bp
Locus specific primer BB79: 5'-GACACAAGTTCAAAATCAGACCAA-3'
2nd locus specific primer 04cr: 5'-TTGAAAGTTGAGGATCTCCTTTGT-3'
   
Note The data concerning the primers refer to the experimental conditions used in the initial analysis of the alleles. In general, we used 8474 in the PCR and 8409 for sequencing. See the confirmation strategy page for details. For genotyping in the context of DUPLO, we used 8409 as T-DNA primer in PCRs. See the DUPLOdb help page for details.

Last Updated on 06.12.2018 16:11