Genotyping and primer details


Genotyping and primer details for 085H10 - At1g13245
Line ID 085H10
Locus At1g13245 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Graphic view of Chr1:4525990 of line 085H10 Chr1:4525990
   
Insertion allele amplicon 3144 - 10lm
T-DNA primer 3144: 5'-GTGGATTGATGTGATATCTCC-3'
Locus specific primer 10lm: 5'-GCTCCTAATTCCCAGAGAGAAAAT-3'
Sequences from confirmation amplicon show confirmation sequences
Genomic end of ins. amplicon Chr1:4526470
   
Insertion allele amplicon 3144 - 15ml
T-DNA primer 3144: 5'-GTGGATTGATGTGATATCTCC-3'
Locus specific primer 15ml: 5'-TCTATAGGTGGAAGAAGACAACCC-3'
Sequences from confirmation amplicon show confirmation sequences
Genomic end of ins. amplicon Chr1:4526423
   
Note The data concerning the primers refer to the experimental conditions used in the initial analysis of the alleles. In general, we used 8474 in the PCR and 8409 for sequencing. See the confirmation strategy page for details. For genotyping in the context of DUPLO, we used 8409 as T-DNA primer in PCRs. See the DUPLOdb help page for details.

Last Updated on 06.12.2018 16:11