Genotyping and primer details


Genotyping and primer details for 206F10 - At1g15400
Line ID 206F10
Locus At1g15400 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Graphic view of Chr1:5296736 of line 206F10 Chr1:5296736
   
Insertion allele amplicon 8474 - 35xg
T-DNA primer 8474: 5'-ATAATAACGCTGCGGACATCTACATTTT-3'
Locus specific primer 35xg: 5'-TATTTATGGATGGGCCTGAGAGTA-3'
Sequences from confirmation amplicon show confirmation sequences
Genomic end of ins. amplicon Chr1:5297854
   
Insertion allele amplicon 8474 - AP15
T-DNA primer 8474: 5'-ATAATAACGCTGCGGACATCTACATTTT-3'
Locus specific primer AP15: 5'-TTCGAAACCCTAGAGGATTAGTCA-3'
Sequences from confirmation amplicon show confirmation sequences
Genomic end of ins. amplicon Chr1:5296060
   
wt amplicon (over InsPos) 33au - AP15
wt amplicon start Chr1:5296060
wt amplicon end Chr1:5297058
wt amplicon size 999 bp
Locus specific primer AP15: 5'-TTCGAAACCCTAGAGGATTAGTCA-3'
2nd locus specific primer 33au: 5'-CGCTTACATTAATTTCCGATGATT-3'
   
Note The data concerning the primers refer to the experimental conditions used in the initial analysis of the alleles. In general, we used 8474 in the PCR and 8409 for sequencing. See the confirmation strategy page for details. For genotyping in the context of DUPLO, we used 8409 as T-DNA primer in PCRs. See the DUPLOdb help page for details.

Last Updated on 06.12.2018 16:11