Genotyping and primer details


Genotyping and primer details for 435H09 - At1g17490
Line ID 435H09
Locus At1g17490 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Graphic view of Chr1:6009017 of line 435H09 Chr1:6009017
   
Insertion allele amplicon 8474 - NA55
T-DNA primer 8474: 5'-ATAATAACGCTGCGGACATCTACATTTT-3'
Locus specific primer NA55: 5'-TCGGTTCGATCTACTCGTTAATCT-3'
Sequences from confirmation amplicon show confirmation sequences
Genomic end of ins. amplicon Chr1:6008527
   
wt amplicon (over InsPos) 08tf - NA55
wt amplicon start Chr1:6008527
wt amplicon end Chr1:6009246
wt amplicon size 720 bp
Locus specific primer NA55: 5'-TCGGTTCGATCTACTCGTTAATCT-3'
2nd locus specific primer 08tf: 5'-AAAGGGGGTGGTTACAGTTCTTAC-3'
   
Note The data concerning the primers refer to the experimental conditions used in the initial analysis of the alleles. In general, we used 8474 in the PCR and 8409 for sequencing. See the confirmation strategy page for details. For genotyping in the context of DUPLO, we used 8409 as T-DNA primer in PCRs. See the DUPLOdb help page for details.

Last Updated on 06.12.2018 16:11