Genotyping and primer details


Genotyping and primer details for 124E07 - At1g17790
Line ID 124E07
Locus At1g17790 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Graphic view of Chr1:6125947 of line 124E07 Chr1:6125947
   
Insertion allele amplicon 8474 - 20ra
T-DNA primer 8474: 5'-ATAATAACGCTGCGGACATCTACATTTT-3'
Locus specific primer 20ra: 5'-AATCTATGACAATCCCTGTGGAAC-3'
Sequences from confirmation amplicon show confirmation sequences
Genomic end of ins. amplicon Chr1:6126378
   
wt amplicon (over InsPos) 19rt - 20ra
wt amplicon start Chr1:6125397
wt amplicon end Chr1:6126378
wt amplicon size 982 bp
Locus specific primer 20ra: 5'-AATCTATGACAATCCCTGTGGAAC-3'
2nd locus specific primer 19rt: 5'-AAAATCGCAATACAGGAATCAATC-3'
   
Note The data concerning the primers refer to the experimental conditions used in the initial analysis of the alleles. In general, we used 8474 in the PCR and 8409 for sequencing. See the confirmation strategy page for details. For genotyping in the context of DUPLO, we used 8409 as T-DNA primer in PCRs. See the DUPLOdb help page for details.

Last Updated on 06.12.2018 16:11