Genotyping and primer details


Genotyping and primer details for 473E12 - At1g18580
Line ID 473E12
Locus At1g18580 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Graphic view of Chr1:6396053 of line 473E12 Chr1:6396053
   
Insertion allele amplicon 8474 - 22wj
T-DNA primer 8474: 5'-ATAATAACGCTGCGGACATCTACATTTT-3'
Locus specific primer 22wj: 5'-TAAACACATAAGCTTTGGCAAGTG-3'
Sequences from confirmation amplicon show confirmation sequences
Genomic end of ins. amplicon Chr1:6396671
   
wt amplicon (over InsPos) 81ww - 22wj
wt amplicon start Chr1:6395512
wt amplicon end Chr1:6396671
wt amplicon size 1160 bp
Locus specific primer 22wj: 5'-TAAACACATAAGCTTTGGCAAGTG-3'
2nd locus specific primer 81ww: 5'-CGTTTGTTACCGTTTATTCGATTT-3'
   
Note The data concerning the primers refer to the experimental conditions used in the initial analysis of the alleles. In general, we used 8474 in the PCR and 8409 for sequencing. See the confirmation strategy page for details. For genotyping in the context of DUPLO, we used 8409 as T-DNA primer in PCRs. See the DUPLOdb help page for details.

Last Updated on 06.12.2018 16:11