Genotyping and primer details


Genotyping and primer details for 727F11 - At1g19700
Line ID 727F11
Locus At1g19700 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Graphic view of Chr1:6810441 of line 727F11 Chr1:6810441
   
Insertion allele amplicon 8474 - 08cz
T-DNA primer 8474: 5'-ATAATAACGCTGCGGACATCTACATTTT-3'
Locus specific primer 08cz: 5'-CCTCTGTACCAAGAAGAACCAGAT-3'
Sequences from confirmation amplicon show confirmation sequences
Genomic end of ins. amplicon Chr1:6809930
   
wt amplicon (over InsPos) 04dq - 08cz
wt amplicon start Chr1:6809930
wt amplicon end Chr1:6810645
wt amplicon size 716 bp
Locus specific primer 08cz: 5'-CCTCTGTACCAAGAAGAACCAGAT-3'
2nd locus specific primer 04dq: 5'-CCTTCATCCGTAAGTGTTTCTTCT-3'
   
Note The data concerning the primers refer to the experimental conditions used in the initial analysis of the alleles. In general, we used 8474 in the PCR and 8409 for sequencing. See the confirmation strategy page for details. For genotyping in the context of DUPLO, we used 8409 as T-DNA primer in PCRs. See the DUPLOdb help page for details.

Last Updated on 06.12.2018 16:11