Genotyping and primer details


Genotyping and primer details for 422A01 - At1g33990
Line ID 422A01
Locus At1g33990 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Graphic view of Chr1:12356591 of line 422A01 Chr1:12356591
   
Insertion allele amplicon 8474 - 27gp
T-DNA primer 8474: 5'-ATAATAACGCTGCGGACATCTACATTTT-3'
Locus specific primer 27gp: 5'-TTTCTAATGGCAAGCAGGTTAAGT-3'
Sequences from confirmation amplicon show confirmation sequences
Genomic end of ins. amplicon Chr1:12356147
   
wt amplicon (over InsPos) 31hs - 27gp
wt amplicon start Chr1:12356147
wt amplicon end Chr1:12356983
wt amplicon size 837 bp
Locus specific primer 27gp: 5'-TTTCTAATGGCAAGCAGGTTAAGT-3'
2nd locus specific primer 31hs: 5'-TCAGTCATATTAAATCCACACCCA-3'
   
Note The data concerning the primers refer to the experimental conditions used in the initial analysis of the alleles. In general, we used 8474 in the PCR and 8409 for sequencing. See the confirmation strategy page for details. For genotyping in the context of DUPLO, we used 8409 as T-DNA primer in PCRs. See the DUPLOdb help page for details.

Last Updated on 06.12.2018 16:11