Genotyping and primer details


Genotyping and primer details for 570A06 - At1g52600
Line ID 570A06
Locus At1g52600 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Graphic view of Chr1:19591327 of line 570A06 Chr1:19591327
   
Insertion allele amplicon 8474 - 52gp
T-DNA primer 8474: 5'-ATAATAACGCTGCGGACATCTACATTTT-3'
Locus specific primer 52gp: 5'-TGAGACAAAGAGAAATGGGAAACT-3'
Sequences from confirmation amplicon show confirmation sequences
Genomic end of ins. amplicon Chr1:19591774
   
wt amplicon (over InsPos) 24hs - 52gp
wt amplicon start Chr1:19590998
wt amplicon end Chr1:19591774
wt amplicon size 777 bp
Locus specific primer 52gp: 5'-TGAGACAAAGAGAAATGGGAAACT-3'
2nd locus specific primer 24hs: 5'-TATTGGTGGTTGGATTATTGGTTT-3'
   
Note The data concerning the primers refer to the experimental conditions used in the initial analysis of the alleles. In general, we used 8474 in the PCR and 8409 for sequencing. See the confirmation strategy page for details. For genotyping in the context of DUPLO, we used 8409 as T-DNA primer in PCRs. See the DUPLOdb help page for details.

Last Updated on 06.12.2018 16:11