Genotyping and primer details


Genotyping and primer details for 434H01 - At1g52670
Line ID 434H01
Locus At1g52670 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Graphic view of Chr1:19616176 of line 434H01 Chr1:19616176
   
Insertion allele amplicon 8474 - 48fr
T-DNA primer 8474: 5'-ATAATAACGCTGCGGACATCTACATTTT-3'
Locus specific primer 48fr: 5'-TTCAGATTTGTGGCTTATGAAGTG-3'
Sequences from confirmation amplicon show confirmation sequences
Genomic end of ins. amplicon Chr1:19616836
   
wt amplicon (over InsPos) 18go - 48fr
wt amplicon start Chr1:19615841
wt amplicon end Chr1:19616836
wt amplicon size 996 bp
Locus specific primer 48fr: 5'-TTCAGATTTGTGGCTTATGAAGTG-3'
2nd locus specific primer 18go: 5'-CGAAGAAGTAGCTGAGCCATTAGT-3'
   
Note The data concerning the primers refer to the experimental conditions used in the initial analysis of the alleles. In general, we used 8474 in the PCR and 8409 for sequencing. See the confirmation strategy page for details. For genotyping in the context of DUPLO, we used 8409 as T-DNA primer in PCRs. See the DUPLOdb help page for details.

Last Updated on 06.12.2018 16:11