Genotyping and primer details


Genotyping and primer details for 003C10 - At1g59890
Line ID 003C10
Locus At1g59890 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Graphic view of Chr1:22049475 of line 003C10 Chr1:22049475
   
Insertion allele amplicon 8474 - 01uv
T-DNA primer 8474: 5'-ATAATAACGCTGCGGACATCTACATTTT-3'
Locus specific primer 01uv: 5'-TTCCTGGTTTTCTGTAGTTCTCGT-3'
Sequences from confirmation amplicon show confirmation sequences
Genomic end of ins. amplicon Chr1:22049886
   
wt amplicon (over InsPos) 66wa - 01uv
wt amplicon start Chr1:22048720
wt amplicon end Chr1:22049886
wt amplicon size 1167 bp
Locus specific primer 01uv: 5'-TTCCTGGTTTTCTGTAGTTCTCGT-3'
2nd locus specific primer 66wa: 5'-ACCAGAAACGATTTGATAATGGAG-3'
   
Note The data concerning the primers refer to the experimental conditions used in the initial analysis of the alleles. In general, we used 8474 in the PCR and 8409 for sequencing. See the confirmation strategy page for details. For genotyping in the context of DUPLO, we used 8409 as T-DNA primer in PCRs. See the DUPLOdb help page for details.

Last Updated on 06.12.2018 16:11