Genotyping and primer details


Genotyping and primer details for 299G11 - At1g67390
Line ID 299G11
Locus At1g67390 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Graphic view of Chr1:25246702 of line 299G11 Chr1:25246702
   
Insertion allele amplicon 8474 - 15uj
T-DNA primer 8474: 5'-ATAATAACGCTGCGGACATCTACATTTT-3'
Locus specific primer 15uj: 5'-TTCTCCATTTTCTACTTTGCGTTT-3'
Sequences from confirmation amplicon show confirmation sequences
Genomic end of ins. amplicon Chr1:25246252
   
wt amplicon (over InsPos) 16vl - 15uj
wt amplicon start Chr1:25246252
wt amplicon end Chr1:25246948
wt amplicon size 697 bp
Locus specific primer 15uj: 5'-TTCTCCATTTTCTACTTTGCGTTT-3'
2nd locus specific primer 16vl: 5'-CAAACATCCACATAACTTCCACAC-3'
   
Note The data concerning the primers refer to the experimental conditions used in the initial analysis of the alleles. In general, we used 8474 in the PCR and 8409 for sequencing. See the confirmation strategy page for details. For genotyping in the context of DUPLO, we used 8409 as T-DNA primer in PCRs. See the DUPLOdb help page for details.

Last Updated on 06.12.2018 16:11