Genotyping and primer details


Genotyping and primer details for 085G10 - At1g74690
Line ID 085G10
Locus At1g74690 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Graphic view of Chr1:28063126 of line 085G10 Chr1:28063126
   
Insertion allele amplicon 3144 - 13wq
T-DNA primer 3144: 5'-GTGGATTGATGTGATATCTCC-3'
Locus specific primer 13wq: 5'-GAACTTTACGGACACTCTTCTTGG-3'
Sequences from confirmation amplicon show confirmation sequences
Genomic end of ins. amplicon Chr1:28062537
   
wt amplicon (over InsPos) 08bj - PA27
wt amplicon start Chr1:28062880
wt amplicon end Chr1:28063649
wt amplicon size 770 bp
Locus specific primer PA27: 5'-GCTGTTAGTTTCTTGATTCCAAGG-3'
2nd locus specific primer 08bj: 5'-ACTGGTTACTTCAAAGGTGGAAGA-3'
   
Note The data concerning the primers refer to the experimental conditions used in the initial analysis of the alleles. In general, we used 8474 in the PCR and 8409 for sequencing. See the confirmation strategy page for details. For genotyping in the context of DUPLO, we used 8409 as T-DNA primer in PCRs. See the DUPLOdb help page for details.

Last Updated on 06.12.2018 16:11