Genotyping and primer details


Genotyping and primer details for 679F03 - At2g01220
Line ID 679F03
Locus At2g01220 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Graphic view of Chr2:125522 of line 679F03 Chr2:125522
   
Insertion allele amplicon 8474 - 36uj
T-DNA primer 8474: 5'-ATAATAACGCTGCGGACATCTACATTTT-3'
Locus specific primer 36uj: 5'-GTCTTTTGACTCTTCGCTGTCTCT-3'
Sequences from confirmation amplicon show confirmation sequences
Genomic end of ins. amplicon Chr2:125021
   
wt amplicon (over InsPos) 28vg - 36uj
wt amplicon start Chr2:125021
wt amplicon end Chr2:125924
wt amplicon size 904 bp
Locus specific primer 36uj: 5'-GTCTTTTGACTCTTCGCTGTCTCT-3'
2nd locus specific primer 28vg: 5'-CACCAAGTATCTCCAACATCCTTT-3'
   
Note The data concerning the primers refer to the experimental conditions used in the initial analysis of the alleles. In general, we used 8474 in the PCR and 8409 for sequencing. See the confirmation strategy page for details. For genotyping in the context of DUPLO, we used 8409 as T-DNA primer in PCRs. See the DUPLOdb help page for details.

Last Updated on 06.12.2018 16:11