Genotyping and primer details


Genotyping and primer details for 213C04 - At2g17420
Line ID 213C04
Locus At2g17420 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Graphic view of Chr2:7564404 of line 213C04 Chr2:7564404
   
Insertion allele amplicon 8474 - 08sf
T-DNA primer 8474: 5'-ATAATAACGCTGCGGACATCTACATTTT-3'
Locus specific primer 08sf: 5'-TTTGTTTAGGTGTTAGGGATAGGG-3'
Sequences from confirmation amplicon show confirmation sequences
Genomic end of ins. amplicon Chr2:7563902
   
wt amplicon (over InsPos) 08sz - 08sf
wt amplicon start Chr2:7563902
wt amplicon end Chr2:7564768
wt amplicon size 867 bp
Locus specific primer 08sf: 5'-TTTGTTTAGGTGTTAGGGATAGGG-3'
2nd locus specific primer 08sz: 5'-TAACAGTTTCCGTGAAGATCGTAG-3'
   
Note The data concerning the primers refer to the experimental conditions used in the initial analysis of the alleles. In general, we used 8474 in the PCR and 8409 for sequencing. See the confirmation strategy page for details. For genotyping in the context of DUPLO, we used 8409 as T-DNA primer in PCRs. See the DUPLOdb help page for details.

Last Updated on 06.12.2018 16:11