Genotyping and primer details


Genotyping and primer details for 409E04 - At2g29065
Line ID 409E04
Locus At2g29065 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Graphic view of Chr2:12486781 of line 409E04 Chr2:12486781
   
Insertion allele amplicon 8474 - 37sj
T-DNA primer 8474: 5'-ATAATAACGCTGCGGACATCTACATTTT-3'
Locus specific primer 37sj: 5'-AGAACCTTCAAGATGAAACAGGAA-3'
Sequences from confirmation amplicon show confirmation sequences
Genomic end of ins. amplicon Chr2:12486436
   
wt amplicon (over InsPos) 05sz - 37sj
wt amplicon start Chr2:12486436
wt amplicon end Chr2:12487297
wt amplicon size 862 bp
Locus specific primer 37sj: 5'-AGAACCTTCAAGATGAAACAGGAA-3'
2nd locus specific primer 05sz: 5'-AATTGGAAAAGTTGTTTTGATTGC-3'
   
Note The data concerning the primers refer to the experimental conditions used in the initial analysis of the alleles. In general, we used 8474 in the PCR and 8409 for sequencing. See the confirmation strategy page for details. For genotyping in the context of DUPLO, we used 8409 as T-DNA primer in PCRs. See the DUPLOdb help page for details.

Last Updated on 06.12.2018 16:11