Genotyping and primer details


Genotyping and primer details for 582E02 - At2g40520
Line ID 582E02
Locus At2g40520 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Graphic view of Chr2:16924777 of line 582E02 Chr2:16924777
   
Insertion allele amplicon 8474 - 11do
T-DNA primer 8474: 5'-ATAATAACGCTGCGGACATCTACATTTT-3'
Locus specific primer 11do: 5'-AGTTTCTGAACTCCCAGCATAAAA-3'
Sequences from confirmation amplicon show confirmation sequences
Genomic end of ins. amplicon Chr2:16925082
   
wt amplicon (over InsPos) 06tf - 11do
wt amplicon start Chr2:16924455
wt amplicon end Chr2:16925082
wt amplicon size 628 bp
Locus specific primer 11do: 5'-AGTTTCTGAACTCCCAGCATAAAA-3'
2nd locus specific primer 06tf: 5'-TATGCATTGGCTATACTCGTCTTG-3'
   
Note The data concerning the primers refer to the experimental conditions used in the initial analysis of the alleles. In general, we used 8474 in the PCR and 8409 for sequencing. See the confirmation strategy page for details. For genotyping in the context of DUPLO, we used 8409 as T-DNA primer in PCRs. See the DUPLOdb help page for details.

Last Updated on 06.12.2018 16:11