Genotyping and primer details


Genotyping and primer details for 080E07 - At2g46210
Line ID 080E07
Locus At2g46210 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Graphic view of Chr2:18978474 of line 080E07 Chr2:18978474
   
Insertion allele amplicon 3144 - 10ax
T-DNA primer 3144: 5'-GTGGATTGATGTGATATCTCC-3'
Locus specific primer 10ax: 5'-AGTACTCGACAAACGAAACACCTT-3'
Sequences from confirmation amplicon show confirmation sequences
Genomic end of ins. amplicon Chr2:18978916
   
Insertion allele amplicon 8474 - AU26
T-DNA primer 8474: 5'-ATAATAACGCTGCGGACATCTACATTTT-3'
Locus specific primer AU26: 5'-AACCGTGTAACAAACTAATCCAGC-3'
Sequences from confirmation amplicon show confirmation sequences
Genomic end of ins. amplicon Chr2:18978053
   
wt amplicon (over InsPos) AU26 - 10ax
wt amplicon start Chr2:18978053
wt amplicon end Chr2:18978916
wt amplicon size 864 bp
Locus specific primer 10ax: 5'-AGTACTCGACAAACGAAACACCTT-3'
2nd locus specific primer AU26: 5'-AACCGTGTAACAAACTAATCCAGC-3'
   
Note The data concerning the primers refer to the experimental conditions used in the initial analysis of the alleles. In general, we used 8474 in the PCR and 8409 for sequencing. See the confirmation strategy page for details. For genotyping in the context of DUPLO, we used 8409 as T-DNA primer in PCRs. See the DUPLOdb help page for details.

Last Updated on 06.12.2018 16:11