Genotyping and primer details


Genotyping and primer details for 067D01 - At3g01260
Line ID 067D01
Locus At3g01260 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Graphic view of Chr3:80925 of line 067D01 Chr3:80925
   
Insertion allele amplicon 8474 - 02tg
T-DNA primer 8474: 5'-ATAATAACGCTGCGGACATCTACATTTT-3'
Locus specific primer 02tg: 5'-CGTGTAGCCAGTAAAAAGGAAAAG-3'
Sequences from confirmation amplicon show confirmation sequences
Genomic end of ins. amplicon Chr3:81304
   
wt amplicon (over InsPos) 04ue - 02tg
wt amplicon start Chr3:80637
wt amplicon end Chr3:81304
wt amplicon size 668 bp
Locus specific primer 02tg: 5'-CGTGTAGCCAGTAAAAAGGAAAAG-3'
2nd locus specific primer 04ue: 5'-ACCCTGTCTTAAGCTCATTGATGT-3'
   
Note The data concerning the primers refer to the experimental conditions used in the initial analysis of the alleles. In general, we used 8474 in the PCR and 8409 for sequencing. See the confirmation strategy page for details. For genotyping in the context of DUPLO, we used 8409 as T-DNA primer in PCRs. See the DUPLOdb help page for details.

Last Updated on 06.12.2018 16:11