Genotyping and primer details


Genotyping and primer details for 109B06 - At3g06620
Line ID 109B06
Locus At3g06620 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Graphic view of Chr3:2064611 of line 109B06 Chr3:2064611
   
Insertion allele amplicon 3144 - EP01
T-DNA primer 3144: 5'-GTGGATTGATGTGATATCTCC-3'
Locus specific primer EP01: 5'-AGTTCCATTTTGGAGATCTGAGTC-3'
Sequences from confirmation amplicon show confirmation sequences
Genomic end of ins. amplicon Chr3:2064059
   
Insertion allele amplicon 8474 - FI61
T-DNA primer 8474: 5'-ATAATAACGCTGCGGACATCTACATTTT-3'
Locus specific primer FI61: 5'-TGTTGATTTATACGTGCATCCCTA-3'
Sequences from confirmation amplicon show confirmation sequences
Genomic end of ins. amplicon Chr3:2065226
   
Note The data concerning the primers refer to the experimental conditions used in the initial analysis of the alleles. In general, we used 8474 in the PCR and 8409 for sequencing. See the confirmation strategy page for details. For genotyping in the context of DUPLO, we used 8409 as T-DNA primer in PCRs. See the DUPLOdb help page for details.

Last Updated on 06.12.2018 16:11