Genotyping and primer details


Genotyping and primer details for 969E09 - At3g10330
Line ID 969E09
Locus At3g10330 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Graphic view of Chr3:3200126 of line 969E09 Chr3:3200126
   
Insertion allele amplicon 8474 - 11ht
T-DNA primer 8474: 5'-ATAATAACGCTGCGGACATCTACATTTT-3'
Locus specific primer 11ht: 5'-AAGCTAGATTCCAAGAAACCATCA-3'
Sequences from confirmation amplicon show confirmation sequences
Genomic end of ins. amplicon Chr3:3200726
   
wt amplicon (over InsPos) 09io - 11ht
wt amplicon start Chr3:3199563
wt amplicon end Chr3:3200726
wt amplicon size 1164 bp
Locus specific primer 11ht: 5'-AAGCTAGATTCCAAGAAACCATCA-3'
2nd locus specific primer 09io: 5'-CCAAAACAATATAGGGCTTCAAAA-3'
   
Note The data concerning the primers refer to the experimental conditions used in the initial analysis of the alleles. In general, we used 8474 in the PCR and 8409 for sequencing. See the confirmation strategy page for details. For genotyping in the context of DUPLO, we used 8409 as T-DNA primer in PCRs. See the DUPLOdb help page for details.

Last Updated on 06.12.2018 16:11