Genotyping and primer details


Genotyping and primer details for 042F09 - At3g15470
Line ID 042F09
Locus At3g15470 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Graphic view of Chr3:5217056 of line 042F09 Chr3:5217056
   
Insertion allele amplicon 8474 - CW11
T-DNA primer 8474: 5'-ATAATAACGCTGCGGACATCTACATTTT-3'
Locus specific primer CW11: 5'-CTTGATGGCTCTAAAAGTTCCTTG-3'
Sequences from confirmation amplicon show confirmation sequences
Genomic end of ins. amplicon Chr3:5217465
   
wt amplicon (over InsPos) 06fd - CW11
wt amplicon start Chr3:5216749
wt amplicon end Chr3:5217465
wt amplicon size 717 bp
Locus specific primer CW11: 5'-CTTGATGGCTCTAAAAGTTCCTTG-3'
2nd locus specific primer 06fd: 5'-ACATATCCGTACTCAGACGATTCC-3'
   
Note The data concerning the primers refer to the experimental conditions used in the initial analysis of the alleles. In general, we used 8474 in the PCR and 8409 for sequencing. See the confirmation strategy page for details. For genotyping in the context of DUPLO, we used 8409 as T-DNA primer in PCRs. See the DUPLOdb help page for details.

Last Updated on 06.12.2018 16:11