Genotyping and primer details


Genotyping and primer details for 764C01 - At3g17970
Line ID 764C01
Locus At3g17970 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Graphic view of Chr3:6150754 of line 764C01 Chr3:6150754
   
Insertion allele amplicon 8474 - AC09
T-DNA primer 8474: 5'-ATAATAACGCTGCGGACATCTACATTTT-3'
Locus specific primer AC09: 5'-CCAATAGCTTTCTGCCATAGTTTC-3'
Sequences from confirmation amplicon show confirmation sequences
Genomic end of ins. amplicon Chr3:6151246
   
Insertion allele amplicon 8474 - AE06
T-DNA primer 8474: 5'-ATAATAACGCTGCGGACATCTACATTTT-3'
Locus specific primer AE06: 5'-GGTGTGCCTATTGTTTATTCACCT-3'
Sequences from confirmation amplicon show confirmation sequences
Genomic end of ins. amplicon Chr3:6150270
   
wt amplicon (over InsPos) AE06 - AC09
wt amplicon start Chr3:6150270
wt amplicon end Chr3:6151246
wt amplicon size 977 bp
Locus specific primer AC09: 5'-CCAATAGCTTTCTGCCATAGTTTC-3'
2nd locus specific primer AE06: 5'-GGTGTGCCTATTGTTTATTCACCT-3'
   
Note The data concerning the primers refer to the experimental conditions used in the initial analysis of the alleles. In general, we used 8474 in the PCR and 8409 for sequencing. See the confirmation strategy page for details. For genotyping in the context of DUPLO, we used 8409 as T-DNA primer in PCRs. See the DUPLOdb help page for details.

Last Updated on 06.12.2018 16:11