Genotyping and primer details


Genotyping and primer details for 659D12 - At3g18160
Line ID 659D12
Locus At3g18160 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Graphic view of Chr3:6221749 of line 659D12 Chr3:6221749
   
Insertion allele amplicon 8474 - 27st
T-DNA primer 8474: 5'-ATAATAACGCTGCGGACATCTACATTTT-3'
Locus specific primer 27st: 5'-GGAAATGACGTTGGTAAAGATTGT-3'
Sequences from confirmation amplicon show confirmation sequences
Genomic end of ins. amplicon Chr3:6222022
   
wt amplicon (over InsPos) 04ti - 27st
wt amplicon start Chr3:6221241
wt amplicon end Chr3:6222022
wt amplicon size 782 bp
Locus specific primer 27st: 5'-GGAAATGACGTTGGTAAAGATTGT-3'
2nd locus specific primer 04ti: 5'-CCCAGTTGAGTTTTTGTAACCTCT-3'
   
Note The data concerning the primers refer to the experimental conditions used in the initial analysis of the alleles. In general, we used 8474 in the PCR and 8409 for sequencing. See the confirmation strategy page for details. For genotyping in the context of DUPLO, we used 8409 as T-DNA primer in PCRs. See the DUPLOdb help page for details.

Last Updated on 06.12.2018 16:11