Genotyping and primer details


Genotyping and primer details for 294D06 - At3g21600
Line ID 294D06
Locus At3g21600 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Graphic view of Chr3:7607718 of line 294D06 Chr3:7607718
   
Insertion allele amplicon 8474 - 50ql
T-DNA primer 8474: 5'-ATAATAACGCTGCGGACATCTACATTTT-3'
Locus specific primer 50ql: 5'-ATGGGATGTTTCGGACCA-3'
Sequences from confirmation amplicon show confirmation sequences
Genomic end of ins. amplicon Chr3:7608364
   
wt amplicon (over InsPos) 02rg - 50ql
wt amplicon start Chr3:7607409
wt amplicon end Chr3:7608364
wt amplicon size 956 bp
Locus specific primer 50ql: 5'-ATGGGATGTTTCGGACCA-3'
2nd locus specific primer 02rg: 5'-CAACACAGCAGCCAAGATATTAAA-3'
   
Note The data concerning the primers refer to the experimental conditions used in the initial analysis of the alleles. In general, we used 8474 in the PCR and 8409 for sequencing. See the confirmation strategy page for details. For genotyping in the context of DUPLO, we used 8409 as T-DNA primer in PCRs. See the DUPLOdb help page for details.

Last Updated on 06.12.2018 16:11