Genotyping and primer details


Genotyping and primer details for 299E05 - At3g21620
Line ID 299E05
Locus At3g21620 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Graphic view of Chr3:7611149 of line 299E05 Chr3:7611149
   
Insertion allele amplicon 8474 - 42qa
T-DNA primer 8474: 5'-ATAATAACGCTGCGGACATCTACATTTT-3'
Locus specific primer 42qa: 5'-GGATCAATGTCAACGTAAAAAGGT-3'
Sequences from confirmation amplicon show confirmation sequences
Genomic end of ins. amplicon Chr3:7610669
   
Note The data concerning the primers refer to the experimental conditions used in the initial analysis of the alleles. In general, we used 8474 in the PCR and 8409 for sequencing. See the confirmation strategy page for details. For genotyping in the context of DUPLO, we used 8409 as T-DNA primer in PCRs. See the DUPLOdb help page for details.

Last Updated on 06.12.2018 16:11