Genotyping and primer details


Genotyping and primer details for 243A01 - At3g23780
Line ID 243A01
Locus At3g23780 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Graphic view of Chr3:8568011 of line 243A01 Chr3:8568011
   
Insertion allele amplicon 8474 - 37wp
T-DNA primer 8474: 5'-ATAATAACGCTGCGGACATCTACATTTT-3'
Locus specific primer 37wp: 5'-TGATATCATTAAGGGCAGGAACAT-3'
Sequences from confirmation amplicon show confirmation sequences
Genomic end of ins. amplicon Chr3:8568928
   
Insertion allele amplicon 3144 - FQ27
T-DNA primer 3144: 5'-GTGGATTGATGTGATATCTCC-3'
Locus specific primer FQ27: 5'-ATCGAAGAATGAGAGAAACGACTC-3'
Sequences from confirmation amplicon show confirmation sequences
Genomic end of ins. amplicon Chr3:8567657
   
Insertion allele amplicon 8474 - HF31
T-DNA primer 8474: 5'-ATAATAACGCTGCGGACATCTACATTTT-3'
Locus specific primer HF31: 5'-TACAACGGTAGATCAGGTGAGATG-3'
Sequences from confirmation amplicon show confirmation sequences
Genomic end of ins. amplicon Chr3:8568438
   
Note The data concerning the primers refer to the experimental conditions used in the initial analysis of the alleles. In general, we used 8474 in the PCR and 8409 for sequencing. See the confirmation strategy page for details. For genotyping in the context of DUPLO, we used 8409 as T-DNA primer in PCRs. See the DUPLOdb help page for details.

Last Updated on 06.12.2018 16:11