Genotyping and primer details


Genotyping and primer details for 180G04 - At3g23840
Line ID 180G04
Locus At3g23840 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Graphic view of Chr3:8612414 of line 180G04 Chr3:8612414
   
Insertion allele amplicon 8474 - 13jr
T-DNA primer 8474: 5'-ATAATAACGCTGCGGACATCTACATTTT-3'
Locus specific primer 13jr: 5'-CTCATTTCTTCAACTTATGGGCTC-3'
Sequences from confirmation amplicon show confirmation sequences
Genomic end of ins. amplicon Chr3:8611808
   
wt amplicon (over InsPos) 22kj - 13jr
wt amplicon start Chr3:8611808
wt amplicon end Chr3:8613015
wt amplicon size 1208 bp
Locus specific primer 13jr: 5'-CTCATTTCTTCAACTTATGGGCTC-3'
2nd locus specific primer 22kj: 5'-CTTCCAAGACAATCTTCCTTTGAC-3'
   
Note The data concerning the primers refer to the experimental conditions used in the initial analysis of the alleles. In general, we used 8474 in the PCR and 8409 for sequencing. See the confirmation strategy page for details. For genotyping in the context of DUPLO, we used 8409 as T-DNA primer in PCRs. See the DUPLOdb help page for details.

Last Updated on 06.12.2018 16:11