Genotyping and primer details


Genotyping and primer details for 701G02 - At3g24120
Line ID 701G02
Locus At3g24120 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Graphic view of Chr3:8707570 of line 701G02 Chr3:8707570
   
Insertion allele amplicon 8474 - 39vf
T-DNA primer 8474: 5'-ATAATAACGCTGCGGACATCTACATTTT-3'
Locus specific primer 39vf: 5'-CAAAATCAAGTCTTTGTTTGACGA-3'
Sequences from confirmation amplicon show confirmation sequences
Genomic end of ins. amplicon Chr3:8706974
   
wt amplicon (over InsPos) 61vo - 39vf
wt amplicon start Chr3:8706974
wt amplicon end Chr3:8707789
wt amplicon size 816 bp
Locus specific primer 39vf: 5'-CAAAATCAAGTCTTTGTTTGACGA-3'
2nd locus specific primer 61vo: 5'-TTGTTGTTCTCGAGACTTGAATTG-3'
   
Note The data concerning the primers refer to the experimental conditions used in the initial analysis of the alleles. In general, we used 8474 in the PCR and 8409 for sequencing. See the confirmation strategy page for details. For genotyping in the context of DUPLO, we used 8409 as T-DNA primer in PCRs. See the DUPLOdb help page for details.

Last Updated on 06.12.2018 16:11