Genotyping and primer details


Genotyping and primer details for 434G03 - At3g25560
Line ID 434G03
Locus At3g25560 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Graphic view of Chr3:9281602 of line 434G03 Chr3:9281602
   
Insertion allele amplicon 8474 - EQ68
T-DNA primer 8474: 5'-ATAATAACGCTGCGGACATCTACATTTT-3'
Locus specific primer EQ68: 5'-GTCAAGCTTACACCGAAGACTACC-3'
Sequences from confirmation amplicon show confirmation sequences
Genomic end of ins. amplicon Chr3:9281088
   
Insertion allele amplicon 3144 - KF55
T-DNA primer 3144: 5'-GTGGATTGATGTGATATCTCC-3'
Locus specific primer KF55: 5'-AATGTTCATCAACAAAATCTTCCC-3'
Sequences from confirmation amplicon show confirmation sequences
Genomic end of ins. amplicon Chr3:9282431
   
wt amplicon (over InsPos) KU43 - EQ68
wt amplicon start Chr3:9281088
wt amplicon end Chr3:9282120
wt amplicon size 1033 bp
Locus specific primer EQ68: 5'-GTCAAGCTTACACCGAAGACTACC-3'
2nd locus specific primer KU43: 5'-CAAGCCAAAACTTATCAGGAACTC-3'
   
wt amplicon (over InsPos) KU43 - EQ68
wt amplicon start Chr3:9281088
wt amplicon end Chr3:9282120
wt amplicon size 1033 bp
Locus specific primer EQ68: 5'-GTCAAGCTTACACCGAAGACTACC-3'
2nd locus specific primer KU43: 5'-CAAGCCAAAACTTATCAGGAACTC-3'
   
Note The data concerning the primers refer to the experimental conditions used in the initial analysis of the alleles. In general, we used 8474 in the PCR and 8409 for sequencing. See the confirmation strategy page for details. For genotyping in the context of DUPLO, we used 8409 as T-DNA primer in PCRs. See the DUPLOdb help page for details.

Last Updated on 06.12.2018 16:11