Genotyping and primer details


Genotyping and primer details for 017A09 - At3g25840
Line ID 017A09
Locus At3g25840 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Graphic view of Chr3:9456647 of line 017A09 Chr3:9456647
   
Insertion allele amplicon 8474 - 69gp
T-DNA primer 8474: 5'-ATAATAACGCTGCGGACATCTACATTTT-3'
Locus specific primer 69gp: 5'-GGCTCTATCCCGTAAATAATCTCC-3'
Sequences from confirmation amplicon show confirmation sequences
Genomic end of ins. amplicon Chr3:9456116
   
Note The data concerning the primers refer to the experimental conditions used in the initial analysis of the alleles. In general, we used 8474 in the PCR and 8409 for sequencing. See the confirmation strategy page for details. For genotyping in the context of DUPLO, we used 8409 as T-DNA primer in PCRs. See the DUPLOdb help page for details.

Last Updated on 06.12.2018 16:11