Genotyping and primer details


Genotyping and primer details for 239H12 - At3g27320
Line ID 239H12
Locus At3g27320 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Graphic view of Chr3:10091665 of line 239H12 Chr3:10091665
   
Insertion allele amplicon 8474 - 17hw
T-DNA primer 8474: 5'-ATAATAACGCTGCGGACATCTACATTTT-3'
Locus specific primer 17hw: 5'-AGAAGTAGGAATTTGCCTGTTTGA-3'
Sequences from confirmation amplicon show confirmation sequences
Genomic end of ins. amplicon Chr3:10092032
   
wt amplicon (over InsPos) 04ji - 17hw
wt amplicon start Chr3:10091392
wt amplicon end Chr3:10092032
wt amplicon size 641 bp
Locus specific primer 17hw: 5'-AGAAGTAGGAATTTGCCTGTTTGA-3'
2nd locus specific primer 04ji: 5'-CACTTGCTTTCTTTCAGGACTTCT-3'
   
Note The data concerning the primers refer to the experimental conditions used in the initial analysis of the alleles. In general, we used 8474 in the PCR and 8409 for sequencing. See the confirmation strategy page for details. For genotyping in the context of DUPLO, we used 8409 as T-DNA primer in PCRs. See the DUPLOdb help page for details.

Last Updated on 06.12.2018 16:11