Genotyping and primer details


Genotyping and primer details for 467G10 - At3g46340
Line ID 467G10
Locus At3g46340 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Graphic view of Chr3:17030854 of line 467G10 Chr3:17030854
   
Insertion allele amplicon 3144 - 70xb
T-DNA primer 3144: 5'-GTGGATTGATGTGATATCTCC-3'
Locus specific primer 70xb: 5'-TCAATCACACGTTGGTTTGTTATT-3'
Sequences from confirmation amplicon show confirmation sequences
Genomic end of ins. amplicon Chr3:17031538
   
Insertion allele amplicon 8474 - EQ76
T-DNA primer 8474: 5'-ATAATAACGCTGCGGACATCTACATTTT-3'
Locus specific primer EQ76: 5'-GTGTACAGGTCGAACTTTTGTTGA-3'
Sequences from confirmation amplicon show confirmation sequences
Genomic end of ins. amplicon Chr3:17030395
   
wt amplicon (over InsPos) KU52 - EQ76
wt amplicon start Chr3:17030395
wt amplicon end Chr3:17031251
wt amplicon size 857 bp
Locus specific primer EQ76: 5'-GTGTACAGGTCGAACTTTTGTTGA-3'
2nd locus specific primer KU52: 5'-GTCAAGATATCCAGGAGTACCAGC-3'
   
Note The data concerning the primers refer to the experimental conditions used in the initial analysis of the alleles. In general, we used 8474 in the PCR and 8409 for sequencing. See the confirmation strategy page for details. For genotyping in the context of DUPLO, we used 8409 as T-DNA primer in PCRs. See the DUPLOdb help page for details.

Last Updated on 06.12.2018 16:11