Genotyping and primer details


Genotyping and primer details for 177B09 - At3g51150
Line ID 177B09
Locus At3g51150 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Graphic view of Chr3:19003530 of line 177B09 Chr3:19003530
   
Insertion allele amplicon 8474 - 04ie
T-DNA primer 8474: 5'-ATAATAACGCTGCGGACATCTACATTTT-3'
Locus specific primer 04ie: 5'-ATTACCTTTTCGATTTGGAGGTCT-3'
Sequences from confirmation amplicon show confirmation sequences
Genomic end of ins. amplicon Chr3:19004037
   
wt amplicon (over InsPos) 02jf - 04ie
wt amplicon start Chr3:19003132
wt amplicon end Chr3:19004037
wt amplicon size 906 bp
Locus specific primer 04ie: 5'-ATTACCTTTTCGATTTGGAGGTCT-3'
2nd locus specific primer 02jf: 5'-GGCTGCGTTTTCTACTTATTTCTG-3'
   
Note The data concerning the primers refer to the experimental conditions used in the initial analysis of the alleles. In general, we used 8474 in the PCR and 8409 for sequencing. See the confirmation strategy page for details. For genotyping in the context of DUPLO, we used 8409 as T-DNA primer in PCRs. See the DUPLOdb help page for details.

Last Updated on 06.12.2018 16:11