Genotyping and primer details


Genotyping and primer details for 356E04 - At3g56080
Line ID 356E04
Locus At3g56080 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Graphic view of Chr3:20812515 of line 356E04 Chr3:20812515
   
Insertion allele amplicon 8474 - 23sj
T-DNA primer 8474: 5'-ATAATAACGCTGCGGACATCTACATTTT-3'
Locus specific primer 23sj: 5'-TTATCAGGAAAAGGAAGCTTTTGA-3'
Sequences from confirmation amplicon show confirmation sequences
Genomic end of ins. amplicon Chr3:20811942
   
wt amplicon (over InsPos) 14ti - 23sj
wt amplicon start Chr3:20811942
wt amplicon end Chr3:20812902
wt amplicon size 961 bp
Locus specific primer 23sj: 5'-TTATCAGGAAAAGGAAGCTTTTGA-3'
2nd locus specific primer 14ti: 5'-GACCAATGATACCATCGATCTCTT-3'
   
Note The data concerning the primers refer to the experimental conditions used in the initial analysis of the alleles. In general, we used 8474 in the PCR and 8409 for sequencing. See the confirmation strategy page for details. For genotyping in the context of DUPLO, we used 8409 as T-DNA primer in PCRs. See the DUPLOdb help page for details.

Last Updated on 06.12.2018 16:11