Genotyping and primer details


Genotyping and primer details for 497A11 - At3g63150
Line ID 497A11
Locus At3g63150 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Graphic view of Chr3:23332350 of line 497A11 Chr3:23332350
   
Insertion allele amplicon 8474 - EQ81
T-DNA primer 8474: 5'-ATAATAACGCTGCGGACATCTACATTTT-3'
Locus specific primer EQ81: 5'-ATCACTCTAGGCATGGTTCAGTTT-3'
Sequences from confirmation amplicon show confirmation sequences
Genomic end of ins. amplicon Chr3:23331817
   
wt amplicon (over InsPos) KU56 - EQ81
wt amplicon start Chr3:23331817
wt amplicon end Chr3:23332714
wt amplicon size 898 bp
Locus specific primer EQ81: 5'-ATCACTCTAGGCATGGTTCAGTTT-3'
2nd locus specific primer KU56: 5'-CCTTCCTCTCTAAATCTCTCCGAT-3'
   
Note The data concerning the primers refer to the experimental conditions used in the initial analysis of the alleles. In general, we used 8474 in the PCR and 8409 for sequencing. See the confirmation strategy page for details. For genotyping in the context of DUPLO, we used 8409 as T-DNA primer in PCRs. See the DUPLOdb help page for details.

Last Updated on 06.12.2018 16:11